The antimicrobial capacity against S. mutans biofilms was investigated using a Calgary biofilm device coated with collagen (CFU and Live/Dead Confocal). Collagenases from Clostridium histolyticum, Porphyromonas gingivalis and S. mutants were used to assess anti-collagenolytic activity.
The biocompatibility of the two compounds with human dental pulp stromal cells (HDPSC) was studied in 3 different donors (ethical approval DREC 251121/HA/336). The findings were published in the International Association for Dental Research (IADR).
AA inhibited the growth of S. mutans and V. parvula, as well as partially inhibited bacterial collagenases (>5 μg/mL). LDT11 (100 μg/mL) inhibited 96% of collagenase activity. AA treatment was associated with odontoblast-like morphology, which was observed after 24 h of treatment.
LDT11 at a concentration of 50 µg/mL had bacteriostatic activity against S. mutans and V. parvula, antimicrobial activity against mature S. mutans biofilms, and anti-collagenolytic activity against bacterial collagenases. It was biocompatible with HDPSCs, stimulating cell proliferation and differentiation.